Determination and stability of some international screening limited drugs in equine blood by LC-MS/MS

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dc.contributor.authorGöktaş, Eylem Funda
dc.contributor.authorKabil, Erol
dc.contributor.authorGüneş, Ertuğrul
dc.contributor.authorKorkmaz, Serol
dc.contributor.authorKadıoğlu, Tuba Asena
dc.contributor.authorCücü, Ayşen Kurt
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-07T07:22:30Z
dc.date.available2021-10-07T07:22:30Z
dc.date.issued2019-09-09
dc.description.abstractStability of some international screening limited therapeutic substances for doping control in equine blood has been investigated with a validated method by using LC-MS/MS after chemical hydrolysis with orthophosphoric acid. Whole blood samples were extracted with a WAX cartridge on auto-SPE. Stability test of the drug substances performed at the same time in working solution and matrix at +4°C, -20°C, +20°C in dark and +20°C in light conditions for 1, 2, 3 and 4 weeks. For short-time stability, the effects of 6 h storage at +55°C was also investigated. Repeated data were statistically analyzed with ANOVA. All substances in working solution were stable at -20°C and +4°C. In the blood matrix, higher temperatures (+20°C) in light and dark caused degradation of substances at the end of four weeks and short-term study (P< 0.05). In conclusion, these international screening limited substances in blood matrix could be unstable related to temperature and storage time, although in working solution they could be mostly stable in various temperatures for four weeks.tr_TR
dc.identifier.endpage372tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue4tr_TR
dc.identifier.startpage363tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.538782tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75286
dc.identifier.volume66tr_TR
dc.language.isotrtr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.538782tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectDoping controltr_TR
dc.subjectEquine bloodtr_TR
dc.subjectLC-MS/MStr_TR
dc.titleDetermination and stability of some international screening limited drugs in equine blood by LC-MS/MStr_TR
dc.typeArticletr_TR

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