Midkine secretion protects Hep3B cells from cadmium induced cellular damage

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dc.contributor.authorYazıhan, Nuray
dc.contributor.authorAtaoğlu, Haluk
dc.contributor.authorAkçil, Ethem
dc.contributor.authorYener, Burcu
dc.contributor.departmentFen Fakültesitr_TR
dc.date.accessioned2020-02-14T13:43:36Z
dc.date.available2020-02-14T13:43:36Z
dc.date.issued2008
dc.description.abstractAim: To evaluate role of midkine secretion during Cadmium (Cd) exposure in the human hepatocyte cell line Hep3B cells. Methods: Different dosages of Cd (0.5-1-5-10 μg/mL) were applied to Hep3B cells and their effects to apoptosis, lactate dehydrogenase (LDH) leakage and midkine secretion were evaluated as time dependent manner. Same experiments were repeated with exogenously applied midkine (250-5000 pg/mL) and/or 5 μg/mL Cd. Results: Cd exposure induced prominent apoptosis and LDH leakage beginning from lower dosages at the 48th h. Cd induced midkine secretion with higher dosages (P < 0.001), (control, Cd 0.5-1-5-10 μg/mL respectively: 1123 ± 73, 1157 ± 63, 1242 ± 90, 1886 ± 175, 1712 ± 166 pg/mL). Exogenous 500-5000 pg/mL midkine application during 5 μg/mL Cd toxicity prevented caspase-3 activation (control, Cd toxicity, 250, 500, 1000, 2500, 5000 pg/mL midkine+ Cd toxicity, respectively: 374 ± 64, 1786 ± 156, 1545 ± 179, 1203 ± 113, 974 ± 116, 646 ± 56, 556 ± 63 cfu) LDH leakage and cell death in Hep3B cells (P < 0.001). Conclusion: Our results showed that midkine secretion from Hep3B cells during Cd exposure protects liver cells from Cd induced cellular damage. Midkine has anti-apoptotic and cytoprotective role during Cd toxicity. Further studies are needed to explain the mechanism of midkine secretion and cytoprotective role of midkine during Cd exposure. Midkine may be a promising theurapatic agent in different toxic hepatic diseases.tr_TR
dc.description.indexScopus
dc.description.indexWos
dc.identifier.endpage080tr_TR
dc.identifier.issn/e-issn1007-9327
dc.identifier.issue1tr_TR
dc.identifier.startpage076tr_TR
dc.identifier.urihttps://doi.org/10.3748/wjg.14.76tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/69518
dc.identifier.volume14tr_TR
dc.language.isoentr_TR
dc.relation.isversionof10.3748/wjg.14.76tr_TR
dc.relation.journalWorld Journal of Gastroenterologytr_TR
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectCadmiumtr_TR
dc.subjectMidkinetr_TR
dc.subjectHepatocytetr_TR
dc.titleMidkine secretion protects Hep3B cells from cadmium induced cellular damagetr_TR
dc.typeArticletr_TR

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