Molecular identification of Paramphistomidae obtained from ruminants in Van province

Simple item page
dc.contributor.authorPadak, Yusuf
dc.contributor.authorKarakuş, Ayşe
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-12T06:20:45Z
dc.date.available2021-10-12T06:20:45Z
dc.date.issued2021-06-30
dc.description.abstractThis study was aimed to identify Paramphistomum species from infected ruminants (sheep and cattle) by molecular methods between March 2018 and September 2018 at Van municipality slaughterhouse. In the research, the rDNA ITS-2 gene region of adult Paramphistomums was amplified by PCR method. Amplicons 399 bp long were viewed in agarose gel. As a result of bidirectional sequence analysis made from PCR amplicons, Paramphistomum leydeni and Calicophoron daubneyi species were identifyed. Amplicon sequences were compared by BLAST with reference sequences from Genbank. Phylogenetic tree was created with the Neighbor-Joining method by using the MEGA 7 program. Genotypes obtained from isolates were compared for exact or closest similarities. In conclusion, C. daubneyi, which was previously detected by morphological methods in Turkey, in this study, it was identified for the first time by using molecular methods. Also in this study, P. leydeni was reported for the first time in Turkey.tr_TR
dc.identifier.endpage228tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue3tr_TR
dc.identifier.startpage223tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.727842tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75395
dc.identifier.volume68tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.727842tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectMolecular identificationtr_TR
dc.subjectParamphistomum spp.tr_TR
dc.subjectPCRtr_TR
dc.titleMolecular identification of Paramphistomidae obtained from ruminants in Van provincetr_TR
dc.typeArticletr_TR

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2.pdf
Size:
650.97 KB
Format:
Adobe Portable Document Format
Description:
Dergi
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Cilt:68 Sayı:03 (2021)